Parallel Plate Flow Chambers  

 

Human Neutrophils Rolling on
Human Endothelial Cells Expressing E-selectin

Parallel plate flow chambers are used in dynamic studies of cell adhesion under well-defined shear forces. Various gasket sizes allow flexibility in experimental design to operate with limited quantities of cells and/or reagents.

(Refer to Protocol 7)

Circular Flow Chamber (for 35mm tissue culture dishes)

31-001

Circular Flow Chamber Kit ($675.00)  

Technical Reference: Improvements to Parallel Plate Flow Chambers to Reduce Reagents and Cellular Requirements, Brown and Larson, BMC Immunology 2001, 2:9

 

Flow Chamber Kit Includes:

Two flow decks
(cast acrylic with different inlet/outlet designs)

8 silicon rubber gaskets (two of each size)
 Flow Width = 0.25 cm, Thickness = 0.005 in
Flow Width = 0.25 cm, Thickness = 0.010 in
Flow Width = 0.50 cm, Thickness = 0.010 in
Flow Width = 1.00 cm, Thickness = 0.010 in

24 polypropylene tubing connectors (six of each)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors

Silastic� laboratory tubing (1/16 in. ID x 10 ft.)
 
Replacement Gaskets and Coverslips for the Circular Flow Chamber
31-002 Four Gaskets  (Flow Width = 0.25 cm, Thickness = 0.005 in) $30.00
31-003 Four Gaskets  (Flow Width = 0.25 cm, Thickness = 0.010 in) $30.00
31-004 Four Gaskets  (Flow Width = 0.50 cm, Thickness = 0.010 in) $30.00
31-005 Four Gaskets  (Flow Width = 1.00 cm, Thickness = 0.010 in) $30.00
31-008 Circular Glass Coverslips (pack of 30, 35 mm, #2) $30.00
 

Rectangular Flow Chamber (for standard microscope slide)

 

31-010

Rectangular Flow Chamber Kit

 $625.00  

 

Flow Chamber Kit Includes:

One flow deck (cast acrylic)

4 silicon rubber gaskets (two of each size)
Flow Width = 1.00 cm, Thickness = 0.005 in
Flow Width = 1.00 cm, Thickness = 0.010 in

24 polypropylene tubing connectors (six of each)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors

Silastic� laboratory tubing (1/16 in. ID x 10 ft.)
 
Replacement Gaskets for the Rectangular Flow Chamber
31-011 Four Gaskets  (Flow Width = 1.0 cm, Thickness = 0.005 in) $30.00
31-012 Four Gaskets  (Flow Width = 1.0 cm, Thickness = 0.010 in) $30.00

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